Journal of Cranio-Maxillo-Facial Surgery
Volume 38, Issue 8 , Pages 580-588 , December 2010

Quantifying migration and polarization of murine mesenchymal stem cells on different bone substitutes by confocal laser scanning microscopy

  • J.C. Roldán

      Affiliations

    • Department of Cranio-Maxillofacial Surgery, University of Regensburg, Germany
    • Corresponding Author InformationJ. Camilo ROLDÁN, MD, DMD, Department of Cranio-Maxillofacial Surgery, University of Regensburg, 93042 Regensburg, Germany.
    • ,
  • E. Chang

      Affiliations

    • Laboratory for Microvascular Surgery and Vascular Tissue Engineering, Stanford University, USA
    • ,
  • M. Kelantan

      Affiliations

    • Laboratory for Microvascular Surgery and Vascular Tissue Engineering, Stanford University, USA
    • ,
  • L. Jazayeri

      Affiliations

    • Laboratory for Microvascular Surgery and Vascular Tissue Engineering, Stanford University, USA
    • ,
  • U. Deisinger

      Affiliations

    • Friedrich-Baur-Research-Institute for Biomaterials, University of Bayreuth, Germany
    • ,
  • R. Detsch

      Affiliations

    • BioCer Entwicklungs-GmbH, Bayreuth, Germany
    • ,
  • T.E. Reichert

      Affiliations

    • Department of Cranio-Maxillofacial Surgery, University of Regensburg, Germany
    • ,
  • G.C. Gurtner

      Affiliations

    • Laboratory for Microvascular Surgery and Vascular Tissue Engineering, Stanford University, USA

Received 19 September 2009 ,Accepted 19 January 2010.

References 

  1. Açil Y, Terheyden H, Dunsche A, Fleiner B, Jepsen S. Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral. J Biomed Mater Res. 2000;51:703–710
  2. Bagambisa FB, Kappert HF, Schilli W. Cellular and molecular biological events at the implant interface. J Craniomaxillofac Surg. 1994;22:12–17
  3. Deisinger U, Stenzel F, Ziegler G. Development of hydroxyapatite ceramics with tailored pore structure. Key Eng Mater. 2004;254:977–980
  4. Evangelista MB, Hsiong SX, Fernandes R, Sampaio P, Kong H, Barrias CC, et al. Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix. Biomaterials. 2007;28:3644–3655
  5. Hollister SJ. Porous scaffold design for tissue engineering. Nat Mater. 2005;4:518–524
  6. Horwitz R, Webb D. Cell migration. Curr Biol. 2003;13:R756–R759
  7. Hamou C, Callaghan MJ, Thangarajah H, Chang E, Jazayeri L, Chang EI, et al. Mesenchymal stem cells can participate in ischemic neovascularization. Plast Reconstr Surg. 2009;123:45S–55S
  8. Huttenlocher A. Cell polarization mechanisms during directed cell migration. Nat Cell Biol. 2005;7:336–337
  9. Jazayeri L, Roldán JC, Kelantan M, Chang E, Fong K, McMahon D, et al. Enhancing neovascularization and subsequent osteogenesis in tissue-engineered bone grafts. Plast Reconstr Surg. 2007;120(S1):34
  10. Kaigler D, Krebsbach PH, Polverini PJ, Mooney DJ. Role of vascular endothelial growth factor in bone marrow stromal cell modulation of endothelial cells. Tissue Eng. 2003;9:95–103
  11. Kaiser JP, Reinmann A, Bruinink A. The effect of topographic characteristics on cell migration velocity. Biomaterials. 2006;27:5230–5241
  12. Kumar PRA, Varma HK, Kumary TV. Rapid and complete cellularization of hydroxyapatite for bone tissue engineering. Acta Biomater. 2005;1:545–552
  13. Maksoud R, Lefevbre L, Heinrich L, Gremillard L, Chevalier J, Hartmann DJ. Preliminary results of in vitro study of cell growth in a 45S5 bioactive glass as bone substitute using scanning electron and confocal microscopies. Key Eng Mater. 2008;361–363:1111–1114
  14. Murphy WL, Simmons CA, Kaigler D, Mooney DJ. Bone regeneration via a mineral substrate and induced angiogenesis. J Dent Res. 2004;83:204–210
  15. Prager-Khoutorsky M, Goncharov I, Rabinkov A, Mirelman D, Geiger B, Bershadsky AD. Allicin inhibits cell polarization, migration and division via its direct effect on microtubules. Cell Motil Cytoskeleton. 2007;64:321–337
  16. Ren F, Smith IO, Case ED, Baumann MJ. Three-dimensional microstructural characterization of porous hydroxyapatite using confocal laser scanning microscopy (CLSM). Int J Appl Ceram Technol. 2005;2:200–211
  17. Ripamonti U, Ramoshebi LN, Matsaba Th, Tasker J, Crooks J, Teare J. Bone induction by BMPs/OPs and related family members in primates: the critical role of delivery systems. J Bone Joint Surg Am. 2001;S83:116–127
  18. Roldán JC, Jepsen S, Miller J, Freitag S, Rueger D, Acil Y, et al. Bone formation in presence of platelet-rich plasma vs. bone morphogenetic protein-7. Bone. 2004;34:80–90
  19. Roldán JC, Jepsen S, Schmidt Ch, Knüppel H, Rueger D, Acil Y, et al. Sinus floor augmentation with simultaneous placement of dental implants in presence of platelet-rich plasma or recombinant bone morphogenetic protein-7. Clin Oral Implants Res. 2004;5:716–723
  20. Roldán JC, Chang E, Jazayeri L, Kelantan M, Deisinger U, Detsch R, et al. Temporal assessment of cells on biomaterials by confocal microscopy. Eur Cell Mater. 2007;S14:90
  21. Roldán JC, Deisinger U, Detsch R, Chang E, Kelantan M, Jazayeri L, et al. A novel HA/TCP ceramic: implant design and bone formation. Eur Cell Mater. 2007;S14:91
  22. Shi X, Sitharaman B, Pham QP, Liang F, Wu K, Edward Billups W, et al. Fabrication of porous ultra-short single-walled carbon nanotube nanocomposite scaffolds for bone tissue engineering. Biomaterials. 2007;28:4078–4090
  23. Smith IO, Ren F, Baumann MJ, Case ED. Confocal laser scanning microscopy as a tool for imaging cancellous bone. J Biomed Mater Res B Appl Biomater. 2006;79B:185–192
  24. Street J, Bao M, de Guzman L, Buntig S, Peale FV, Ferrara N, et al. Vascular endothelial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. Proc Natl Acad Sci USA. 2002;23:9656–9661
  25. Sutter W, Stein E, Koehn J, Schmidl C, Lezaic V, Ewers R, et al. Effect of different biomaterials on the expression pattern of the transcription factor Ets2 in bone-like constructs. J Craniomaxillofac Surg. 2009;37:263–271
  26. Thangarajah H, Vial IN, Chang E, El-Ftesi S, Januszyk M, Chang EI, et al. IFATS series: adipose stromal cells adopt a proangiogenic phenotype under the influence of hypoxia. Stem Cells. 2009;27:266–274
  27. Unger RE, Sartoris A, Peters K, Motta A, Migliaresi C, Kunkel M, et al. Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials. Biomaterials. 2007;28:3965–3976
  28. Vicente-Manzanares M, Webb DJ, Horwitz AR. Cell migration at a glance. J Cell Sci. 2005;118:4917–4919
  29. Warnke PH, Bolte H, Schünemann K, Nitsche T, Sivananthan S, Sherry E, et al. Endocultivation: does delayed application of BMP improve intramuscular heterotopic bone formation?. J Craniomaxillofac Surg. 2010;38:54–59
  30. Weinzierl K, Hemprich K, Frerich B. Bone engineering with adipose tissue derived stromal cells. J Craniomaxillofac Surg. 2006;34:466–471

PII: S1010-5182(10)00016-8

doi: 10.1016/j.jcms.2010.01.004

Journal of Cranio-Maxillo-Facial Surgery
Volume 38, Issue 8 , Pages 580-588 , December 2010

Article Tools

* Image Usage & Resolution